Shiitake Mushroom, Lentinula edodes 10cc Live Liquid Culture Syringe

A:We have found that most people use anywhere from a 20-50 quart tote However, this will depend on the measurements of the tote. Are you are breaking the kit up or leaving it as one solid cake? If breaking it up, you need to decide how thick you want your substrate layer to be. 2-3 inches is pretty standard. If you measure the length, width and height of the tote you will find out how may cubic inches it is. Then measure the length and width and depth you want your substrate layer to be. Our 5 LB kits measure 7.5'' high x 6'' wide x 4'' deep which is 180 cubic inches, or 3.17 quarts. From this you can determine what size tote you will need.

A:Our compost works best when inoculated with colonized grain spawn. If you just inject this bag with spores you will more than likely not see any growth. Spores need some kind of nutritive substance like grain to take ahold of and establish mycelium growth first. Start by injecting your spores or liquid culture into a sterilized grain medium (we have this for sale in our store), then once the grain is colonized you can transfer it to the compost, then once the compost is colonized the mushrooms will grow right out of the bag or you can transfer them to some kind of fruiting chamber, tote or terrarium.

A:Yes, we ship it in that way for two reasons. First, we have less bags burst in shipping when tightly packed and shrink wrapped. Second, having the least amount of air in each bag before we seal then ensures they are able to be stored if necessary. Before using any shipped substrate, allow a day or two for it to reach room temperature and loosen the material in the bags before inoculation. This allows more air flow durning your grow.

A:This is all made possible because of sterilization.The grain is stored in sealed bags with almost all oxygen removed. Therefor they are kept in almost a suspended like state (think of food stored in a food saver bag). They key is the oxygen. Molds and bacterias that break down the grain and cause contaminates need air to live. Without it they will not grow. When you remove the bags of grain from their packaging, unroll them and expose the filter patch, the bag will begin to let in outside air and breath through this filter. This is what allows a desired culture to grow once introduced to the substrate.

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